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Related Experiment Video

Updated: Jul 5, 2026

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

Magnetization transfer based contrast for imaging denatured collagen.

Amir Harel¹, Uzi Eliav, Solange Akselrod

¹Department of Medical Physics, Tel Aviv University, Tel Aviv, Israel.

Journal of Magnetic Resonance Imaging : JMRI

|April 22, 2008

Summary

Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI) methods effectively detect thermal collagen denaturation. Double quantum filtering (DQF) and T(2) filtering show high sensitivity and contrast enhancement for denatured collagen.

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Area of Science:

Biophysics
Materials Science
Biochemistry

Background:

Collagen denaturation alters its structural and dynamic properties.
Understanding these changes is crucial for various biological and medical applications.
Sensitive detection methods are needed to quantify collagen denaturation.

Purpose of the Study:

To evaluate the sensitivity of various Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI) techniques to thermal collagen denaturation.
To compare the effectiveness of different NMR/MRI parameters in distinguishing native from denatured collagen.

Main Methods:

Collagen type I was thermally denatured at temperatures ranging from 50-100°C.
Tested NMR methods included T(2) filter methods (Goldman-Shen, Edzes-Samulski), magnetization transfer contrast (MTC), double quantum filtering (DQF), and high-resolution spectroscopy.
MRI contrasts derived from these NMR methods were compared.

Main Results:

Double quantum filtering (DQF) effectively assessed spins with high dipolar interactions.
Magnetization transfer ratio (MTR) and the product of magnetization transfer rate and water proton longitudinal relaxation time (k(w)T(1w)) were sensitive parameters.
Aliphatic residue content also indicated denaturation.
Edzes-Samulski (ES) and DQF methods yielded the highest contrast between native and denatured collagen.

Conclusions:

T(2) filtering and DQF-based NMR methods are highly sensitive to the degree of thermal collagen denaturation.
These methods significantly improve the contrast, enabling better differentiation between native and denatured collagen states.