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Published on: March 16, 2017
Prostaglandin E1 transport into rat intestinal cryptic cell line IEC-6
Kohji Hirata1, Kumiko Mabashi, Toshiharu Horie
1Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences Chiba University, 1-8-1 Inohana, Chuoku-ku, Chiba 260-8675, Japan.
Abstract:
Administration of antitumor drugs such as 5-fluorouracil and methotrexate to rats causes intestinal injury. The intestinal injury was reported to be prevented by coadministration of prostaglandin E1 analogues to rats. IEC-6 cell is a useful in vitro model to study the cytoprotective effect in enterocytes, For its purpose, it is important to know whether or not prostaglandins are taken up into IEC-6 cells. The transport of prostaglandin E1 into IEC-6 cells was investigated, using [3H]-prostaglandin E1. Prostaglandin E1 was found to be transported into IEC-6 cells by the passive diffusion mechanism.
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