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Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

Advances in cell culture process development: tools and techniques for improving cell line development and process

Susan T Sharfstein1

  • 1Department of Chemical and Biological Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, New York 12180, USA. sharfs@rpi.edu

Biotechnology Progress
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Cell culture process development addresses increased product demands and shorter timelines. Techniques like automated flow cytometry and epigenetic analysis improve cell line production and product quality.

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Area of Science:

  • Biotechnology and Bioprocessing
  • Chemical Engineering
  • Cell Biology

Background:

  • Cell culture process development faces challenges in meeting rising product demands and compressed timelines.
  • Maintaining robust process performance, product quality, and adherence to Quality by Design (QbD) principles are critical.
  • The American Chemical Society (ACS) BIOT division convened to discuss these challenges.

Purpose of the Study:

  • To explore strategies for enhancing cell culture process development.
  • To address the need for increased productivity and improved product quality.
  • To present advancements in cell line development and analytical techniques.

Main Methods:

  • Presentations focused on understanding process condition effects on productivity and quality.
  • Development and application of improved production cell lines were discussed.
  • Advanced analytical techniques including automated flow cytometry, transcriptional and epigenetic analyses, and glycoform analysis were featured.

Main Results:

  • Various techniques were presented to optimize cell culture processes.
  • Methods for analyzing cell lines and culture systems were highlighted.
  • Novel approaches for glycoform analysis were introduced.

Conclusions:

  • Optimizing process conditions is key to enhancing productivity and quality.
  • Advancements in cell line engineering are crucial for biopharmaceutical manufacturing.
  • Integrated analytical strategies are essential for robust cell culture development.