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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jul 5, 2026

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

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Published on: February 12, 2020

[Function of matrix metalloprotenase-2 by RNA interference].

Xiao-Hui Hu¹, Lei Fan, Chang-Geng Ruan

¹Jiangsu Institute of Hematology, The First Hospital Affiliated to Suzhou University, Suzhou 215006, Jiangsu Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi

|April 23, 2008

Summary

Matrix metalloproteinase-2 (MMP-2) significantly impacts endothelial cell migration, invasion, and angiogenesis, but not proliferation. MMP-2 regulates the cell cycle via Rb, cyclinD1, and PCNA gene expression.

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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Context:

Matrix metalloproteinase-2 (MMP-2) is implicated in various cellular processes.
Understanding MMP-2's role in endothelial cells is crucial for vascular biology.
This study investigates the specific functions of MMP-2 in EAhy926 endothelial cells.

Purpose:

To elucidate the effects of MMP-2 on endothelial cell proliferation, migration, invasion, angiogenesis, and cell cycle.
To evaluate the impact of MMP-2 inhibition using small interfering RNA (siRNA).
To analyze changes in cell cycle regulatory gene expression following MMP-2 knockdown.

Summary:

MMP-2 inhibition via siRNA did not affect EAhy926 cell proliferation.
MMP-2 knockdown significantly reduced endothelial cell migration and invasion induced by type IV collagen and fibronectin.
Angiogenesis was reduced by 58.9% post-MMP-2 interference, and cell cycle analysis showed a G1 phase increase and S/G2 phase decrease.

Impact:

MMP-2 plays a critical role in endothelial cell migration, invasion, and angiogenesis.
MMP-2 regulates the cell cycle, influencing Rb, cyclin D1, and PCNA gene expression.
Findings highlight MMP-2 as a potential therapeutic target in angiogenesis-related diseases.