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Related Experiment Video

Updated: Jul 5, 2026

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

Current techniques for single-cell lysis.

Robert B Brown¹, Julie Audet

¹Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, 164 College Street, Room 407, Toronto, Ontario, Canada.

Journal of the Royal Society, Interface

|April 23, 2008

Summary

Choosing the right single-cell lysis method is crucial for accurate analysis. Various techniques exist, each with pros and cons, impacting downstream processing and data quality.

Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Single-cell analysis requires precise manipulation due to small sample volumes.
Effective cell lysis is a critical prerequisite for downstream molecular analysis.

Purpose of the Study:

To review and compare different single-cell lysis methods.
To guide the selection of optimal lysis techniques based on experimental needs.

Main Methods:

Overview of optical, acoustic, mechanical, electrical, and chemical lysis strategies.
Discussion of ultrafast lysis techniques (laser, high voltage).
Consideration of detergent-based methods for native or denatured protein collection.

Main Results:

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Each lysis method presents unique advantages and limitations.
Method selection is contingent upon downstream processing requirements.
Cellular state (adherent/suspension) and protein integrity influence technique choice.

Conclusions:

Careful selection of single-cell lysis techniques is essential for reliable data acquisition.
Optimizing lysis protocols enhances the accuracy and utility of single-cell analyses.
Understanding method-specific characteristics improves experimental design in single-cell studies.