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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jul 5, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

miRDB: a microRNA target prediction and functional annotation database with a wiki interface.

¹Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. xwang@radonc.wustl.edu

RNA (New York, N.Y.)

|April 23, 2008

Summary

miRDB is a new online database for microRNA (miRNA) target prediction and functional annotation. It offers a community-driven approach to cataloging miRNA functions, aiding bioinformatics research.

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Last Updated: Jul 5, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

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Published on: May 1, 2021

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

Bioinformatics
Molecular Biology
Genomics

Background:

MicroRNAs (miRNAs) are crucial regulators of gene expression, controlling thousands of gene targets.
The functional importance of miRNAs necessitates comprehensive data organization, posing a significant bioinformatics challenge.
Existing miRNA functional databases struggle to keep pace with the rapid accumulation of knowledge.

Purpose of the Study:

To introduce miRDB, a novel online database for miRNA target prediction and functional annotation.
To provide a user-friendly platform for accessing and contributing to miRNA functional data.
To focus on mature miRNAs as the primary functional units of gene regulation.

Main Methods:

Development of a new bioinformatics algorithm for miRNA target prediction.
Creation of a flexible web interface for retrieving prediction results.
Implementation of a wiki-based editing system for community-driven functional annotation.

Main Results:

miRDB provides accurate miRNA target predictions generated by a novel algorithm.
Functional annotations in miRDB emphasize mature miRNAs, the active molecules in gene regulation.
An interactive, community-annotated catalog of miRNA functions is established.

Conclusions:

miRDB addresses the challenge of organizing and presenting miRNA function data.
The database facilitates miRNA research through accessible target prediction and annotation.
miRDB represents a significant advancement in creating a dynamic, community-updated resource for miRNA functional genomics.