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Updated: Jul 5, 2026
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In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
Published on: October 25, 2024
Human Th17 cell clones and natural immune responses
Sho Matsushita1, Takehiro Higashi
1Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama, Japan. shomat@saitama-med.ac.jp
Summary
Certain polysaccharides, like beta-glucan, can induce Th17 cells, a distinct immune response. This review explores methods for studying Th17 cells and their role in human immunity.
Area of Science:
- Immunology
- Cell Biology
Background:
- Immunomodulators like lipopolysaccharides (LPS) and forskolin direct dendritic cells (DCs) to induce Th1 and Th2 immune responses, respectively.
- Recent research indicates that specific polysaccharides, such as beta-glucan from Candida albicans, possess Th17-inducing activity.
- Understanding the induction of T helper 17 (Th17) cells is crucial for developing novel immunotherapies.
Purpose of the Study:
- To review the mechanisms by which certain polysaccharides induce Th17 cell responses.
- To introduce effective methods for establishing human Th17 cell clones.
- To discuss the in vivo relevance of Th17 responses in humans.
Main Methods:
- Mixed Lymphocyte Reaction (MLR) assays to assess T cell proliferation and activation.
- Cyclic adenosine monophosphate (cAMP) level measurements to understand signaling pathways.
- Transcriptome analysis of human Th subpopulations to identify differential gene expression.
- Establishment of human Th17 cell clones for detailed study.
Main Results:
- Beta-glucan and potentially other polysaccharides can act as Th17 adjuvants.
- Specific methods allow for the generation and characterization of human Th17 cell clones.
- Transcriptome analysis provides insights into the molecular signature of human Th subpopulations.
Conclusions:
- Certain polysaccharides represent a novel class of Th17-inducing immunomodulators.
- The presented methods facilitate the study of human Th17 cell responses.
- Further investigation into the in vivo role of Th17 cells is warranted for clinical applications.
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