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Related Experiment Video

Updated: Jul 5, 2026

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Published on: January 7, 2019

Translation regulatory factor RBM3 is a proto-oncogene that prevents mitotic catastrophe.

S M Sureban¹, S Ramalingam, G Natarajan

¹Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73126, USA.

|April 23, 2008

Summary

The RNA-binding protein RBM3 is upregulated in human tumors and promotes cell proliferation and anchorage-independent growth. Downregulating RBM3 halts tumor growth and induces mitotic catastrophe, identifying it as a proto-oncogene.

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Area of Science:

Molecular Biology
Cancer Research
Cell Biology

Background:

RNA-binding proteins regulate mRNA stability and translation.
RBM3, a translation regulatory protein, is implicated in cancer biology.

Purpose of the Study:

To investigate the role of RBM3 in human tumors and its impact on cell growth and transformation.
To elucidate the molecular mechanisms by which RBM3 influences cancer progression.

Main Methods:

RBM3 expression analysis in human tumors.
Overexpression and knockdown studies in cell lines (NIH3T3, SW480, HCT116).
Soft agar assays, xenograft studies, apoptosis assays, and Western blotting.

Main Results:

RBM3 is upregulated in human tumors, particularly colorectal tumors.
RBM3 overexpression promotes cell proliferation and anchorage-independent growth.
RBM3 knockdown inhibits tumor xenograft growth, induces mitotic catastrophe, and affects mRNA translation of key genes (COX-2, IL-8, VEGF).

Conclusions:

RBM3 functions as a proto-oncogene, driving cellular transformation and tumor progression.
RBM3 is essential for cell cycle progression and mitosis.
RBM3 regulates the stability and translation of specific oncogenic transcripts.