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Related Experiment Video

Updated: Jul 5, 2026

Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule

Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule

Published on: May 9, 2025

Cell models for studying renal physiology.

M Bens¹, A Vandewalle

¹INSERM U773, Centre de Recherche Biomédicale Bichat Beaujon, 75870 Cedex 18, Paris, France.

Pflugers Archiv : European Journal of Physiology

|April 23, 2008

Summary

Cultured renal tubule cell lines, developed over 20 years, offer powerful in vitro models for studying kidney tubule functions. These immortalized cell lines, often using simian virus 40 large T antigen (Tag), are crucial for analyzing ion transport and cell-specific biochemical processes.

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Area of Science:

Nephrology
Cell Biology
Molecular Biology

Background:

Development of cultured renal tubule cell lines over 20 years.
Cell lines provide powerful in vitro systems for analyses.
Immortalized cell lines utilize hybrid gene constructs, often simian virus 40 large T antigen (Tag).

Purpose of the Study:

Summarize cellular immortalization strategies.
Review commonly used human, rabbit, rat, and mouse tubule cell lines.
Overview use of immortalized mouse tubule cell lines for in vitro analyses.

Main Methods:

Establishment of immortalized cell lines using hybrid gene constructs (e.g., Tag).
Development of transgenic mice for cell line derivation.
Microdissection or immunoselection of renal proximal, distal, and collecting duct tubules.

Main Results:

Variety of cultured renal tubule cell lines available from different segments.
Established permanent cell lines from microdissected or immunoselected tubules.
Utilized Tag gene for cellular immortalization.

Conclusions:

Cultured renal tubule cell lines are invaluable for in vitro analysis of tubule segment-specific functions.
Immortalized cell lines facilitate study of ion transporters and membranous channels.
Primary cultures from transgenic mouse models offer advantages for studying physiopathology.