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Molecular epidemiological study of HEV-B enteroviruses involved in the increase in meningitis cases occurred in Spain

María Cabrerizo1, Juan E Echevarria, Irene González

  • 1National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. mcabrerizo@isciii.es

Journal of Medical Virology
|April 23, 2008
PubMed

Insights

Echovirus 30 was the most frequent cause of aseptic meningitis in Spain in 2006. Molecular typing identified a new Echovirus 30 lineage, aiding rapid epidemic response for enterovirus infections.

Area of Science:

  • Virology
  • Epidemiology
  • Molecular Biology

Background:

  • Human enteroviruses, particularly enterovirus B species, are major causes of aseptic meningitis and central nervous system infections.
  • Molecular methods are crucial for identifying enterovirus serotypes and tracking their epidemiology.
  • A significant rise in enterovirus meningitis cases occurred in Spain during the spring of 2006.

Purpose of the Study:

  • To genotype 138 enteroviruses from clinical samples of patients with aseptic meningitis and other neurological conditions in Spain.
  • To identify the predominant enterovirus serotypes involved in the 2006 meningitis outbreak.
  • To perform phylogenetic analysis of Echovirus 30 strains to understand their evolutionary relationships.

Main Methods:

  • Direct molecular typing of enteroviruses from clinical samples.
  • Amplification and sequencing of the VP1 (viral protein 1) region.
  • Phylogenetic analysis of sequenced viral strains.

Main Results:

  • Echovirus 30 was the most prevalent serotype identified, followed by echovirus 6, 9, 13, 18, enterovirus 75, coxsackievirus A9, echovirus 11, 14, 29, 4, and coxsackievirus B4 and B5.
  • Phylogenetic analysis revealed that Spanish Echovirus 30 strains from 2006 formed a distinct lineage, separate from previously described sequences.
  • Molecular typing enabled rapid identification of serotypes during the epidemic alert.

Conclusions:

  • Echovirus 30 is the most common enterovirus serotype associated with aseptic meningitis in Spain.
  • Direct molecular typing of clinical samples is effective for rapid serotype identification during outbreaks.
  • Phylogenetic analysis of the 3'-VP1 region is a valuable tool for studying enterovirus epidemiology.

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