Related Experiment Video
Updated: Jul 5, 2026

12:34
DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Methylation-specific PCR
1The Johns Hopkins Oncology Center, Baltimore, Maryland, USA.
Current Protocols in Human Genetics
|April 23, 2008
Abstract:
Methylation-specific PCR is a rapid method used to determine the methylation status of DNA. Not only does methylation affect the expression of genes in normal cells, but it is now known that disease processes such as cancer can result in abnormal DNA methylation patterns. Methylation-specific PCR can be used to investigate imprinted genes, to assess human tumors for clonality by studying genes inactivated on the X chromosome, and to examine abnormally methylated CpG islands in neoplasia.

