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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
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Oligonucleotide arrays for expression monitoring

M C Byrne1, M Z Whitley, M T Follettie

  • 1Genetics Institute, Cambridge, Massachusetts, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed

Abstract:

This unit provides protocols for the amplification and labeling of mRNA (and the necessary controls) for hybridization to oligonucleotide arrays. It also describes methods for processing and normalizing the raw gene expression data in preparation for clustering and further analysis.

Keywords:
transcriptomicsmRNA labelinggenomic data analysishybridization protocols

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