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Differential display of mRNA by PCR.

F J Livesey1, S P Hunt

  • 1Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary

This study details mRNA differential display, a polymerase chain reaction (PCR)-based method to find genes with varying expression levels between cells. It covers gene identification, reamplification, cloning, and preparing RNA samples for accurate PCR analysis.

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