Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
Cancer02:18

Cancer

Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The use of central volaris forearm flap in oral and maxillofacial reconstruction]

Shanghai kou qiang yi xue = Shanghai journal of stomatology·2004
Same author

Observation of a re-entrant kinetic glass transition in a micellar system with temperature-dependent attractive interaction.

The European physical journal. E, Soft matter·2004
Same author

Antibody-mediated endothelial cell damage via nitric oxide.

Current pharmaceutical design·2004
Same author

Primer Design Assistant (PDA): A web-based primer design tool.

Nucleic acids research·2003
Same author

[The development of cerebral circulation analyzer].

Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation·2003
Same author

Gene sequencing for identification of Paragonimus eggs from a human case.

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases·2003
Same journal

Resolving Breakpoints of Chromosomal Rearrangements at the Nucleotide Level Using Sanger Sequencing.

Current protocols in human genetics·2020
Same journal

Informed Consent for Genetic and Genomic Research.

Current protocols in human genetics·2020
Same journal

A Guide to Using ClinTAD for Interpretation of DNA Copy Number Variants in the Context of Topologically Associated Domains.

Current protocols in human genetics·2020
Same journal

The AD Knowledge Portal: A Repository for Multi-Omic Data on Alzheimer's Disease and Aging.

Current protocols in human genetics·2020
Same journal

A Practical Guide for Structural Variation Detection in the Human Genome.

Current protocols in human genetics·2020
Same journal

Identification and Genotyping of Transposable Element Insertions From Genome Sequencing Data.

Current protocols in human genetics·2020
See all related articles

Related Experiment Video

Updated: Jul 5, 2026

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

Methods for cancer gene therapy.

Y L Kwong1, S H Chen, S L Woo

  • 1Baylor College of Medicine, Houston, Texas, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

This study details methods for testing therapeutic gene vectors in vitro and in vivo. It also covers generating tumor models and assessing anti-tumor immunity following cytokine gene therapy.

More Related Videos

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

Related Experiment Videos

Last Updated: Jul 5, 2026

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

Area of Science:

  • Immunology
  • Gene Therapy
  • Oncology

Background:

  • Cytokine gene therapy holds promise for cancer treatment.
  • Effective delivery and assessment of therapeutic gene vectors are crucial.
  • Developing reliable preclinical models is essential for evaluating treatment efficacy.

Purpose of the Study:

  • To describe methods for in vitro and in vivo testing of therapeutic gene vectors.
  • To outline techniques for generating both subcutaneous and orthotopic murine tumor models.
  • To present methods for evaluating anti-tumor immunity post-cytokine gene therapy.

Main Methods:

  • In vitro testing of therapeutic gene vectors.
  • In vivo administration and assessment of therapeutic vectors.
  • Generation of subcutaneous and orthotopic murine tumor models.
  • Assessment of anti-tumor immunity using cytokine gene therapy.

Main Results:

  • Established protocols for in vitro and in vivo gene vector evaluation.
  • Successful generation of syngeneic murine tumor models (subcutaneous and orthotopic).
  • Defined methods for measuring anti-tumor immune responses.

Conclusions:

  • The described methods provide a framework for preclinical evaluation of gene therapy strategies.
  • These techniques facilitate the study of anti-tumor immunity in relevant models.
  • This work supports the advancement of cytokine gene therapy for cancer treatment.