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Related Concept Videos

Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...

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Treating Hepatobiliary Cancer: The Immunologic Approach.

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Modulation of tumor eIF4E by antisense inhibition: A phase I/II translational clinical trial of ISIS 183750-an antisense oligonucleotide against eIF4E-in combination with irinotecan in solid tumors and irinotecan-refractory colorectal cancer.

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Methods for retrovirus-mediated gene transfer to tumor cells.

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Related Experiment Video

Updated: Jul 5, 2026

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
12:43

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Published on: January 6, 2014

Cancer vaccines.

T F Greten1, E M Jaffee

  • 1Johns Hopkins University School of Medicine, Baltimore, Maryland.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

This study details using retroviral vectors for gene transfer in tumor cells, including methods for vaccine stability, storage, and characterization. It also provides guidance for animal experiments and clinical settings.

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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

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Related Experiment Videos

Last Updated: Jul 5, 2026

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
12:43

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Published on: January 6, 2014

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
09:15

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Therapy

Background:

  • Retroviral vectors are key tools for gene transfer.
  • Gene transfer into tumor cells can be used for cancer vaccines.
  • Assessing vaccine stability and efficacy is crucial.

Purpose of the Study:

  • To describe methods for gene transfer into tumor cells using retroviral vectors.
  • To outline procedures for evaluating the resulting cancer vaccine.
  • To provide guidance for preclinical and clinical applications.

Main Methods:

  • Gene transfer into primary tumor cultures and cell lines using retroviral vectors.
  • Assaying retroviral vector stability and titering.
  • Characterizing gene-transferred tumor cells.
  • Frozen storage protocols for transduced tumor cells.
  • Testing transferred gene expression.

Main Results:

  • Established protocols for gene transfer and characterization of tumor cells.
  • Methods for ensuring vaccine stability and proper storage.
  • Guidelines for in vivo animal studies and clinical observations.

Conclusions:

  • Retroviral vectors offer a viable method for creating cancer vaccines through gene transfer.
  • Comprehensive protocols are provided for vaccine development, characterization, and application.
  • The described methods support the progression of gene therapy cancer vaccines from laboratory to clinical settings.