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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

PCR

PCR

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: Jul 5, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Manual methods for PCR-based forensic DNA analysis.

D H Bing¹, F R Bieber

¹Genomics Collaborative, Cambridge, Massachusetts, USA.

Current Protocols in Human Genetics

|April 23, 2008

Summary

This summary is machine-generated.

This study details validated Polymerase Chain Reaction (PCR)-based methods for identifying genetic variations, including sequence and length polymorphisms. These techniques are crucial for forensic science and genetic testing applications.

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Area of Science:

Molecular Biology
Forensic Genetics

Background:

Polymerase Chain Reaction (PCR) is a fundamental technique in molecular biology.
Detecting genetic variations like polymorphisms is essential for various applications, including forensics.

Purpose of the Study:

To provide validated PCR-based methods for detecting sequence and length polymorphisms.
To describe specific protocols for forensic PCR systems.

Main Methods:

Reverse dot blot method for sequence polymorphism detection.
Electrophoresis of PCR products for length polymorphism analysis (VNTRs and STRs).
Multiplex amplification of autosomal STRs and amelogenin alleles.

Main Results:

Detailed protocols for amplification and detection of D1S80 VNTR locus.
Description of multiplex PCR for autosomal STRs and amelogenin alleles.
Methods for electrophoretic separation on denaturing polyacrylamide gels.

Conclusions:

The unit offers comprehensive PCR-based strategies for genetic polymorphism analysis.
Includes support protocols for gel recording, reagent quality control, and test interpretation.
These methods are applicable to forensic investigations and genetic research.