Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PCR methods of genotyping.

T J Hudson1, C D Clark, M Gschwend

  • 1Whitehead Institute, Cambridge, Massachusetts, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mucous membrane pemphigoid of the oral lichen type: a retrospective analysis of 16 cases.

Journal of the European Academy of Dermatology and Venereology : JEADV·2019
Same author

Ice stream activity scaled to ice sheet volume during Laurentide Ice Sheet deglaciation.

Nature·2016
Same author

Genetic and epigenetic profiling of CLL disease progression reveals limited somatic evolution and suggests a relationship to memory-cell development.

Blood cancer journal·2015
Same author

Maxwell's equations-based dynamic laser-tissue interaction model.

Computers in biology and medicine·2013
Same author

Higher-order wide-angle split-step spectral method for non-paraxial beam propagation.

Optics express·2013
Same author

Genome variation and personalized cancer medicine.

Journal of internal medicine·2013
Same journal

Resolving Breakpoints of Chromosomal Rearrangements at the Nucleotide Level Using Sanger Sequencing.

Current protocols in human genetics·2020
Same journal

Informed Consent for Genetic and Genomic Research.

Current protocols in human genetics·2020
Same journal

A Guide to Using ClinTAD for Interpretation of DNA Copy Number Variants in the Context of Topologically Associated Domains.

Current protocols in human genetics·2020
Same journal

The AD Knowledge Portal: A Repository for Multi-Omic Data on Alzheimer's Disease and Aging.

Current protocols in human genetics·2020
Same journal

A Practical Guide for Structural Variation Detection in the Human Genome.

Current protocols in human genetics·2020
Same journal

Identification and Genotyping of Transposable Element Insertions From Genome Sequencing Data.

Current protocols in human genetics·2020
See all related articles

This study details methods for analyzing PCR products to determine Short Tandem Repeat Polymorphism (STLP) genotypes. Techniques include radioactive labeling, autoradiography, silver staining, and chemiluminescent detection for precise genetic analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Short Tandem Repeat Polymorphism (STLP) analysis is crucial for genetic fingerprinting and disease research.
  • Accurate and efficient genotyping methods are essential for various molecular biology applications.

Purpose of the Study:

  • To present and compare multiple protocols for Short Tandem Repeat Polymorphism (STLP) genotyping.
  • To evaluate different detection methods for PCR-amplified STLP markers, including radioactive and non-radioactive approaches.

Main Methods:

  • Radioactive labeling of PCR products followed by denaturing polyacrylamide gel electrophoresis and autoradiography.
  • Silver staining technique for detecting PCR-amplified STLP markers after electrophoresis.
  • Non-radioactive chemiluminescent detection using end-labeled probes after membrane transfer of separated PCR products.

Related Experiment Videos

Main Results:

  • Successful determination of STLP genotypes using radioactive labeling and autoradiography.
  • Effective visualization of STLP markers via silver staining.
  • Demonstration of chemiluminescent detection for sensitive and specific STLP analysis.

Conclusions:

  • Multiple validated methods exist for STLP genotyping, offering flexibility in laboratory settings.
  • Both radioactive and non-radioactive techniques provide reliable results for STLP analysis.
  • The chemiluminescent method offers a sensitive alternative for detecting STLP markers.