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Multicolor Fluorescence In Situ Hybridization (FISH) approaches for simultaneous analysis of the entire human genome

C Lee1, W Rens, F Yang

  • 1Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...