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Updated: Jul 5, 2026

Denaturing Gradient Gel Electrophoresis (DGGE)
Published on: February 25, 2007
Detection of mutations by denaturing gradient gel electrophoresis
A L Børresen-Dale1, E Hovig, B Smith-Sørensen
1The Norwegian Radium Hospital, Oslo, Norway.
Abstract:
This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.
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