Related Experiment Video
Updated: Jul 5, 2026

09:48
Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
Published on: October 9, 2013
Single-sperm typing
Sigbjorn Lien1, Joanna Szyda, Esther P Leeflang
1Agricultural University of Norway, Aas, Norway.
Current Protocols in Human Genetics
|April 23, 2008
Abstract:
This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.

