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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

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Related Experiment Video

Updated: Jul 5, 2026

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
09:48

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

Single-sperm typing.

Sigbjorn Lien1, Joanna Szyda, Esther P Leeflang

  • 1Agricultural University of Norway, Aas, Norway.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

This study details methods for isolating sperm DNA and analyzing genetic recombination using techniques like simple sequence-length polymorphisms (SSLP) and single nucleotide polymorphisms (SNP) in single cells.

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Related Experiment Videos

Last Updated: Jul 5, 2026

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
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Published on: October 9, 2013

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A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Area of Science:

  • Genetics
  • Molecular Biology
  • Reproductive Science

Background:

  • Understanding genetic recombination is crucial for studying inheritance and genetic diversity.
  • Analyzing recombination at the single-cell level provides high-resolution insights into genetic processes.

Purpose of the Study:

  • To present established and novel protocols for sperm DNA isolation and genetic analysis.
  • To facilitate the study of recombination events over short genetic distances.

Main Methods:

  • Sperm isolation using two distinct methods.
  • Amplification of simple sequence-length polymorphisms (SSLP) and single nucleotide polymorphisms (SNP) from single cells.
  • Whole genome amplification of single cells using primer extension preamplification (PEP).
  • Statistical analysis of sperm-typing recombination data.
  • Allele-specific PCR for studying recombination in total sperm DNA.

Main Results:

  • Protocols for single-cell sperm DNA isolation and amplification are presented.
  • Methods allow for the analysis of genetic recombination using SSLP and SNP markers.
  • Newer techniques enable the study of recombination over kilobase distances.

Conclusions:

  • The presented protocols offer robust methods for single-cell genetic analysis in sperm.
  • These techniques are valuable for investigating genetic recombination and its patterns.
  • The study contributes to advancing the understanding of genetic variation and inheritance.