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Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Published on: January 7, 2019
Construction of replication-defective herpes simplex virus vectors.
William F Goins1, Peggy Marconi, David Krisky
1University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Current Protocols in Human Genetics
|April 23, 2008
Summary
Herpes simplex virus (HSV)-based vectors offer novel gene replacement therapies for nervous system diseases. Researchers developed methods to create replication-defective HSV vectors for targeted gene delivery to neurons.
Area of Science:
- Neuroscience
- Molecular Biology
- Gene Therapy
Background:
- Gene therapy holds promise for treating nervous system diseases.
- Herpes simplex virus (HSV)-based vectors are suitable for neuronal gene delivery.
Purpose of the Study:
- To develop methods for creating gene transfer vectors using HSV.
- To enable gene replacement therapies for neurological disorders.
Main Methods:
- Deletion of HSV-1 immediate-early (IE) gene functions.
- Introduction of foreign genes into the HSV-1 genome via homologous recombination.
- Generation of cell lines complementing essential gene deletion mutants.
Main Results:
- Successful creation of replication-defective HSV recombinants.
- Insertion of foreign DNA sequences into viral genomes.
- Established protocols for vector preparation, including virus stock production and titration.
Conclusions:
- Developed a robust method for generating HSV-based gene therapy vectors.
- These vectors are suitable for delivering therapeutic genes to the central and peripheral nervous systems.
- The described protocols facilitate the development of novel treatments for neurological diseases.
