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Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
Published on: August 15, 2019
Comparative bioinformatics for mouse and human genes: getting started
1Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Abstract:
The primary goal of this unit is to describe methods for using online bioinformatic resources to identify data pertaining to specific mouse genes or genomic regions, from the perspective of a researcher who has identified a human gene(s) of interest and wishes to obtain some basic information about the orthologous murine gene-such as the mouse genomic sequence containing the gene, cDNA sequence and exon/intron structure, and conserved noncoding regions that may indicate regulatory elements. Basic Protocol 1 describes how to obtain mouse gene sequences via Ensembl. Basic Protocol 2 describes a sequence homology search for locating a mouse ortholog of a human gene using the UCSC genome browser. Basic Protocols 3 and 4 provide instructions for performing comparative analysis of large human and mouse genomics sequences, using the PipMaker (Schwartz et al., 2000) and VISTA (Mayor et al., 2000) online tools.
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