GrailEXP and Genome Analysis Pipeline for genome annotation
Edward C Uberbacher1, Doug Hyatt, Manesh Shah
1Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Current Protocols in Human Genetics
|April 23, 2008
Summary
The Gene Recognition and Analysis Internet Link (GRAIL) system helps identify protein-coding regions in DNA. It uses XGRAIL and genQuest tools for gene modeling and homolog searches.
Area of Science:
- Bioinformatics
- Computational Biology
- Genomics
Background:
- The Gene Recognition and Analysis Internet Link (GRAIL) is a widely utilized system for analyzing DNA sequences.
- Accurate identification of protein-coding regions is crucial for understanding gene function.
Purpose of the Study:
- To describe the utilization of XGRAIL and genQuest client-server applications.
- To demonstrate the application of these tools in locating exons, developing gene models, and searching for homologous sequences.
- To provide instructions for obtaining the necessary software via anonymous FTP.
Main Methods:
- Utilizing the XGRAIL client-server application for DNA sequence analysis.
- Employing genQuest for database searches of homologous sequences.
- Describing a support protocol for software acquisition.
Main Results:
- The study outlines the practical application of GRAIL, XGRAIL, and genQuest for gene identification.
- The described methods facilitate the development of gene models from anonymous DNA sequences.
- The protocol enables users to access and utilize these computational tools.
Conclusions:
- GRAIL, XGRAIL, and genQuest are effective tools for analyzing DNA sequences and identifying protein-coding potential.
- The described client-server applications streamline the process of gene modeling and homolog searching.
- Accessible protocols enhance the usability of these bioinformatics resources for researchers.
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