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Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

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A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
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EGF receptor testing for non-small cell lung carcinomas.

Juan-Sebastian Saldivar1, Zhenbin Chen, Steve Sommer

  • 1City of Hope National Medical Center, Duarte, California, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary

This protocol details EGFR kinase domain mutation analysis for non-small cell lung cancer (NSCLC) treatment selection. It aids in identifying patients likely to respond to EGFR inhibitors, improving targeted therapy outcomes.

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Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
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Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Published on: August 11, 2017

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is a prevalent malignancy globally, with significant morbidity and mortality.
  • Emerging targeted therapies, specifically small molecule inhibitors of the Epidermal Growth Factor Receptor (EGFR) kinase domain, offer new treatment avenues for advanced NSCLC.
  • EGFR genotyping is crucial for predicting patient response to these targeted therapies.

Purpose of the Study:

  • To provide a detailed protocol for performing mutation analysis on the EGFR kinase domain.
  • To establish a reliable method for identifying EGFR mutations associated with responsiveness to EGFR inhibitors in NSCLC patients.
  • To ensure accurate and sensitive detection of somatic mutations within tumor tissue.

Main Methods:

  • Microdissection of tumor tissue from pathological slides.
  • DNA extraction and digestion from isolated tumor cells.
  • Polymerase chain reaction (PCR) amplification and sequencing of key EGFR gene segments.
  • Data interpretation focusing on mutation identification and validation.

Main Results:

  • The protocol is designed to maximize the detection of clinically relevant somatic mutations in the EGFR kinase domain.
  • Built-in redundancies aim to minimize allele dropout, ensuring reliable results.
  • Successful implementation allows for precise genotyping to guide therapeutic decisions.

Conclusions:

  • This protocol provides a robust method for EGFR mutation analysis in NSCLC.
  • Accurate genotyping facilitates personalized treatment strategies using EGFR inhibitors.
  • The described methodology supports improved patient outcomes through targeted therapy selection.