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Updated: Jul 5, 2026

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Published on: January 17, 2019
Selective isolation of mammalian genes by TAR cloning
Natalay Kouprina1, Vladimir Larionov
1National Cancer Institute, Bethesda, Maryland, USA.
Abstract:
Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.
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