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Serial analysis of gene expression (SAGE): experimental method and data analysis

Seth Blackshaw1, Brad St Croix, Kornelia Polyak

  • 1Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
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Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
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