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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...

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Updated: Jul 5, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Serial analysis of gene expression (SAGE): experimental method and data analysis.

Seth Blackshaw1, Brad St Croix, Kornelia Polyak

  • 1Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary

Serial analysis of gene expression (SAGE) offers a method to profile gene activity using DNA tags. This technique provides absolute RNA measurements for reliable, shareable gene expression data.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression profiling is crucial for understanding cellular function.
  • Existing methods may have limitations in accuracy or data comparability.

Purpose of the Study:

  • To provide a detailed protocol for Serial Analysis of Gene Expression (SAGE).
  • To enable comprehensive gene expression profiling from small biological samples.

Main Methods:

  • SAGE generates short DNA tags from specific positions in cDNAs.
  • These tags are sufficient to identify individual transcripts.
  • The protocol is applicable to as few as 100,000 cells or 1 microg of total RNA.

Main Results:

  • SAGE generates absolute RNA abundance levels, not relative ones.
  • This allows for reliable comparison of data across different studies and laboratories.
  • Comprehensive gene expression profiles can be generated.

Conclusions:

  • SAGE is a robust method for detailed gene expression analysis.
  • The absolute nature of SAGE data enhances its utility and shareability.
  • Associated software facilitates data analysis and clustering.