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Updated: Jul 5, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Large-scale BAC clone restriction digest fingerprinting
Carrie A Mathewson1, Jacqueline E Schein, Marco A Marra
1Canada's Michael Smith Genome Sciences Center Vancouver, British Columbia, Canada.
Abstract:
Restriction digest fingerprinting is a common method for characterizing large insert genomic clones, e.g., bacterial artificial chromosome (BAC), P1 artificial chromosome (PAC) and Fosmid clones. This clone fingerprinting method has been widely applied in the construction of clone-based physical maps, which have been used as positional cloning resources as well as to support directed and genome-wide sequencing efforts. This unit describes a robust, large-scale procedure for generation of agarose gel-based clone fingerprints from BAC clones.
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