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Related Experiment Video

Updated: May 9, 2026

Driving Simulation in the Clinic: Testing Visual Exploratory Behavior in Daily Life Activities in Patients with Visual Field Defects

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Genotyping using the TaqMan assay.

Lester Hui¹, Terrye DelMonte, Koustubh Ranade

¹Bristol-Myers Squibb, Princeton, New Jersey, USA.

Current Protocols in Human Genetics

|April 23, 2008

Summary

The TaqMan assay is a PCR-based method for genotyping single nucleotide polymorphisms (SNPs). It uses allele-specific probes for accurate results without post-PCR processing.

Area of Science:

Molecular Biology
Genetics

Background:

Single nucleotide polymorphisms (SNPs) are common genetic variations.
Accurate SNP genotyping is crucial for various research applications.

Purpose of the Study:

To describe the 5'-nuclease allelic discrimination assay (TaqMan assay) for SNP genotyping.
To provide guidance on probe and primer design and PCR conditions.

Main Methods:

Utilizes the polymerase chain reaction (PCR) for amplification.
Employs two allele-specific fluorescent probes with quenchers.
Enables detection of both alleles in a single reaction tube.

Main Results:

The TaqMan assay allows for real-time genotyping during PCR.

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Genotypes are determined without the need for post-PCR processing.

Offers a streamlined approach compared to other genotyping methods.

Conclusions:

The TaqMan assay is an efficient and versatile method for SNP genotyping.
The described methods facilitate accurate and rapid genetic analysis.