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Updated: Jul 5, 2026

Preliminary Validation of Stereotaxic Injection Coordinates via Cryosectioning
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High precision stereotaxic surgery in mice.

J Athos1, D R Storm

  • 1University of Washington, Seattle, Washington, USA.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary

This study details precise mouse brain cannulation and microinjection methods using a novel stereotaxic frame. Protocols cover device construction and surgical procedures for accurate central nervous system delivery.

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Area of Science:

  • Neuroscience
  • Surgical Techniques
  • Animal Models

Background:

  • Accurate delivery of substances to the central nervous system is crucial for research.
  • Existing stereotaxic methods may lack the required precision for specific applications.
  • Development of advanced tools is needed to improve microinjection accuracy in mice.

Purpose of the Study:

  • To provide detailed protocols for cannulation and site-specific central microinjection in mice.
  • To describe the construction of essential components for microinjection procedures.
  • To introduce a high-precision stereotaxic frame for enhanced experimental control.

Main Methods:

  • Utilizing a newly developed high-precision stereotaxic frame for mouse brain procedures.
  • Implementing detailed protocols for surgical cannulation.
  • Describing the fabrication of cannulae, wire plugs, and injection needles.

Main Results:

  • Successful demonstration of site-specific central microinjection in mice.
  • Established protocols for reproducible cannulation and injection procedures.
  • Construction guidelines for specialized microinjection apparatus.

Conclusions:

  • The described protocols and apparatus enable precise central microinjection in mice.
  • This method enhances the reliability of studies involving targeted central nervous system delivery.
  • The high-precision stereotaxic frame facilitates advanced neuroscientific research.

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