Related Experiment Video
Updated: Jan 25, 2026
06:01
Fluorescence Lifetime Macro Imager for Biomedical Applications
Published on: April 7, 2023
1.1K
Theory and application of fluorescence microscopy.
Current Protocols in Neuroscience
|April 23, 2008
Summary
Fluorescence microscopy is crucial in biology, particularly neurobiology, due to advances in fluorescent probes and imaging systems. This review details key components and techniques for effective fluorescence microscopy applications.
Area of Science:
- Biology
- Neurobiology
- Microscopy
Background:
- Fluorescence microscopy is increasingly vital in biological and neurobiological research.
- Its importance stems from advancements in fluorescent molecular probes and imaging technologies.
Purpose of the Study:
- To provide a comprehensive overview of fluorescence microscopy techniques and components.
- To cover essential aspects for researchers utilizing fluorescence microscopy.
Main Methods:
- Review of fluorescent molecular probes.
- Discussion of filters, filter sets, and multiband filters for multidye fluorescence.
- Examination of light sources, microscope objectives, and image resolution (point-spread function).
- Outline of general immunolabeling steps.
Main Results:
- Detailed explanation of fluorescent molecular probes and their applications.
- Analysis of optical components and their impact on image quality.
- Guidance on achieving optimal resolution and signal detection.
- Practical steps for immunolabeling protocols.
Conclusions:
- The integration of advanced probes and imaging systems enhances fluorescence microscopy.
- Understanding these components is critical for successful biological and neurobiological imaging.
- This overview serves as a guide for researchers in the field.
Related Concept Videos
02:35Band Theory
17.1K
When two or more atoms come together to form a molecule, their atomic orbitals combine and molecular orbitals of distinct energies result. In a solid, there are a large number of atoms, and therefore a large number of atomic orbitals that may be combined into molecular orbitals. These groups of molecular orbitals are so closely placed together to form continuous regions of energies, known as the bands.
The energy difference between these bands is known as the band gap.
Conductor, Semiconductor,...
The energy difference between these bands is known as the band gap.
Conductor, Semiconductor,...
17.1K
01:39Chromosomal Theory of Inheritance
59.8K
In 1866, Gregor Mendel published the results of his pea plant breeding experiments, providing evidence for predictable patterns in the inheritance of physical characteristics. The significance of his findings was not immediately recognized. In fact, the existence of genes was unknown at the time. Mendel referred to hereditary units as “factors.”
59.8K
02:31Scientific Laws and Theories
87.6K
Scientific Laws
87.6K
00:56Attribution Theory
13.8K
Behavior is a product of both the situation (e.g., cultural influences, social roles, and the presence of bystanders) and of the person (e.g., personality characteristics). Subfields of psychology tend to focus on one influence or behavior over others. Situationism is the view that our behavior and actions are determined by our immediate environment and surroundings. In contrast, dispositionism holds that our behavior is determined by internal factors (Heider, 1958).
13.8K
02:59The Atomic Theory of Matter
127.2K
The earliest recorded discussion of the basic structure of matter comes from ancient Greek philosophers. Leucippus and Democritus argued that all matter was composed of small, finite particles that they called atomos, meaning “indivisible.” Later, Aristotle and others came to the conclusion that matter consisted of various combinations of the four “elements” — fire, earth, air, and water — and could be infinitely divided. Interestingly, these philosophers...
127.2K
02:06Social Exchange Theory
39.5K
We have discussed why we form relationships, what attracts us to others, and different types of love. But what determines whether we are satisfied with and stay in a relationship? One theory that provides an explanation is social exchange theory. According to social exchange theory, we act as naïve economists in keeping a tally of the ratio of costs and benefits of forming and maintaining a relationship with others (Rusbult & Van Lange, 2003).
39.5K