Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Cellular Differentiation

Cellular Differentiation

How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultraviolet absorbance at 260 and 280 nm in RNA measurement is dependent on measurement solution.

International journal of molecular medicine·2000

Same author

Gastric acid secretion in dogs in response to combinations of beer, ethanol and peptone meal--the role of endogenous gastrin.

Alimentary pharmacology & therapeutics·2000

Same author

Combinatorial approach to development of peptides that recognize DNA tetraloops.

Nucleic acids symposium series·2000

Same author

Effects of nasal continuous positive airway pressure on awake ventilatory responses to hypoxia and hypercapnia in patients with obstructive sleep apnea.

The Tohoku journal of experimental medicine·2000

Same author

Selective action of a CCK-B/gastrin receptor antagonist, S-0509, on pentagastrin-, peptone meal- and beer-stimulated gastric acid secretion in dogs.

Alimentary pharmacology & therapeutics·2000

Same author

A new concept of tumor promotion by tumor necrosis factor-alpha, and cancer preventive agents (-)-epigallocatechin gallate and green tea--a review.

Cancer detection and prevention·2000

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

Differentiation of embryonic stem cells.

¹National Institute of Bioscience and Human Technology, Ibaraki, Japan.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This study details a method for differentiating embryonic stem (ES) cells into neural cells. The protocol involves forming embryoid bodies (EBs) and culturing them in serum-free conditions, offering a scalable approach for neural cell production.

Area of Science:

Developmental Biology
Stem Cell Research
Neuroscience

Background:

Embryonic stem (ES) cells possess pluripotency, mirroring the inner cell mass of blastocysts.
Neural differentiation protocols are crucial for regenerative medicine and disease modeling.

Purpose of the Study:

To present a reproducible method for differentiating ES cells into neural lineages.
To establish conditions for maintaining differentiated neural cells and inducing further maturation.

Main Methods:

Aggregation of ES cells to form embryoid bodies (EBs) on non-adhesive surfaces.
Attachment of EBs to a substrate for differentiation in serum-free medium.
Culture of differentiated cells with basic fibroblast growth factor (bFGF) or glial monolayers for maturation.

More Related Videos

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

Related Experiment Videos

Last Updated: Jul 5, 2026

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

Main Results:

Successful differentiation of ES cells into neural cells in a serum-free environment.
Maintenance of differentiated cells through several passages.
Induction of postmitotic neurons via glial co-culture.

Conclusions:

The described method provides a robust protocol for generating neural cells from ES cells.
This approach supports the expansion and directed differentiation of neural progeny.
The protocol includes essential steps for feeder and glial cell preparation.