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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Immunoprecipitation

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Related Experiment Video

Updated: Jul 5, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

H M Cooper¹, Y Paterson

¹Ludwig Institute for Cancer Research, Melbourne, Australia.

Current Protocols in Neuroscience

|April 23, 2008

Summary

This summary is machine-generated.

This study details the production of polyclonal antisera, which are antibodies crucial for immunoprecipitation and immunoblotting. The methods cover generating specific antibodies in various common laboratory animals like rabbits and mice.

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Last Updated: Jul 5, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

Immunology
Biotechnology

Background:

Antibodies are essential serum immunoglobulins with specific antigen-binding capabilities.
Polyclonal antibodies are highly valuable reagents for techniques such as immunoprecipitation and immunoblotting.

Purpose of the Study:

To describe the production of polyclonal antisera specific for protein antigens.
To provide a protocol for generating polyclonal antibodies in common laboratory animals.

Main Methods:

Immunization of rabbits, rats, mice, and hamsters with protein antigens.
Collection and processing of blood to obtain serum.
Characterization of antibody specificity for immunoprecipitation and immunoblotting applications.

Main Results:

Successful generation of polyclonal antisera in multiple animal models.
Demonstrated utility of produced antisera in immunoprecipitation and immunoblotting assays.

Conclusions:

The described methods provide a reliable approach for producing specific polyclonal antibodies.
These antibodies are valuable tools for various immunological research applications.