Production of polyclonal antisera

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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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H M Cooper1, Y Paterson

  • 1Ludwig Institute for Cancer Research, Melbourne, Australia.

Current Protocols in Neuroscience
|April 23, 2008
PubMed

Antibodies are serum immunoglobulins with binding specificity for particular antigens, and polyclonal antibodies are particularly valuable for immunoprecipitation and immunoblotting. In this unit, the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters is described. A support protocol is included for preparing serum from blood.

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