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Related Experiment Videos

Microdialysis in rodents.

A C Thompson1, T S Shippenberg

  • 1NIH/NIDA Intramural Research Program, Baltimore, Maryland, USA.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Microdialysis is a key in vivo sampling method for measuring substances in blood and tissue. This technique utilizes a semipermeable membrane for substance analysis and infusion in research settings.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Pharmacology

Background:

  • Microdialysis is a well-established technique for in vivo substance measurement in biological fluids and tissues.
  • It enables the quantification of various molecules, including neurotransmitters, hormones, and pharmaceuticals.
  • Recent advancements allow for the infusion of substances into the central nervous system.

Purpose of the Study:

  • To describe the construction of different microdialysis probe designs (concentric, side-by-side, horizontal).
  • To detail the surgical implantation and in vitro/in vivo application of microdialysis in rodents.
  • To provide methods for quantitative microdialysis.

Main Methods:

  • Utilizing a semipermeable dialysis membrane perfused with physiological fluid.

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  • Employing passive diffusion for molecule migration across the membrane based on concentration gradients.
  • Collecting diffused molecules for subsequent quantification.

    • Main Results:

    • Construction methods for concentric, side-by-side, and modified horizontal microdialysis probes are detailed.
    • Surgical procedures for probe implantation in rats and mice are outlined.
    • Protocols for in vitro and in vivo microdialysis in rodents are provided.

    Conclusions:

    • This unit provides a comprehensive guide to microdialysis probe construction and application.
    • It facilitates in vivo and in vitro substance analysis and infusion in rodent models.
    • The described quantitative techniques enhance the utility of microdialysis in research.