Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different degree of heart failure.

Naunyn-Schmiedeberg's archives of pharmacology·2014
Same author

Increased CD39 nucleotidase activity on microparticles from patients with idiopathic pulmonary arterial hypertension.

PloS one·2012
Same author

Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base.

BMC bioinformatics·2007
Same author

International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers.

Pharmacological reviews·2007

Related Experiment Video

Updated: Jul 5, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Analyzing radioligand binding data.

Harvey Motulsky1, Richard Neubig

  • 1Graphpad Software, San Diego, California, USA.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary

Radioligand binding assays offer valuable data for receptor studies, drug discovery, and understanding signaling mechanisms. This guide covers binding theory, data analysis, and nonlinear regression for accurate interpretation.

Area of Science:

  • Pharmacology
  • Biochemistry
  • Neuroscience

Background:

  • Radioligand binding assays are versatile tools in biological research.
  • They provide insights into receptor characteristics and interactions.
  • Understanding the theoretical basis and data analysis is crucial for reliable results.

Purpose of the Study:

  • To review the fundamental theory of radioligand binding.
  • To explain methods for analyzing experimental binding data.
  • To introduce nonlinear regression for curve fitting in binding studies.

Main Methods:

  • Review of radioligand binding assay principles.
  • Explanation of data analysis techniques.
  • Introduction to nonlinear regression and curve fitting.

More Related Videos

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

Related Experiment Videos

Last Updated: Jul 5, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Published on: March 12, 2019

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

Main Results:

  • Radioligand binding assays facilitate receptor regulation studies.
  • They are instrumental in drug discovery through compound screening.
  • Autoradiography enables receptor localization investigations.
  • Binding data analysis using nonlinear regression ensures accurate interpretation.

Conclusions:

  • Radioligand binding assays are essential for diverse research applications.
  • Proper data analysis, including nonlinear regression, is key to extracting meaningful biological information.
  • This unit provides a foundational understanding for conducting and interpreting binding experiments.