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Related Experiment Video

Updated: Jul 5, 2026

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

Ballistic labeling with fluorescent dyes and indicators.

Joshua L Morgan¹, Rachel O L Wong

¹University of Washington, Seattle, Washington, USA.

Current Protocols in Neuroscience

|April 23, 2008

Summary

Ballistic dye delivery rapidly labels large neuronal populations in live or fixed tissue. This method propels dye-coated particles into cells for studying neuronal architecture and function.

Area of Science:

Neuroscience
Cell Biology
Biophysics

Background:

Neuronal cell labeling is crucial for understanding nervous system structure and function.
Existing methods may have limitations in speed or scale for labeling large neuronal populations.

Purpose of the Study:

To provide detailed protocols for a ballistic dye delivery method for neuronal cell labeling.
To enable rapid labeling of extensive neuronal populations in both live and fixed tissues.

Main Methods:

Coating tungsten particles with fluorescent or indicator dyes.
Propelling dye-coated particles into cells and tissue using a pulse of pressurized helium.
Detailed protocols for particle preparation and delivery are provided.

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Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Published on: February 12, 2014

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Last Updated: Jul 5, 2026

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Published on: February 12, 2014

Main Results:

The ballistic approach allows for rapid labeling of large neuronal populations.
Effective labeling achieved in both live and fixed tissue samples.
The method facilitates the study of neuronal architecture and function.

Conclusions:

Ballistic dye delivery offers a significant advantage for high-throughput neuronal labeling.
The protocols presented provide a valuable resource for neuroscientists.
The method's advantages and limitations are discussed, with technical guidance offered.