Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genres and typologies of standard paediatric service public funding model provisions for speech-language pathology management: A scoping review.

Health policy OPEN·2026
Same author

Aligned or misaligned: Are public funding models for speech-language pathology reflecting recommended evidence? An exploratory survey of Australian speech-language pathologists.

Health policy OPEN·2024
Same author

High prevalence of slight and mild hearing loss across mid-life: a cross-sectional national Australian study.

Public health·2019
Same author

Does a brief, behavioural intervention, delivered by paediatricians or psychologists improve sleep problems for children with ADHD? Protocol for a cluster-randomised, translational trial.

BMJ open·2017
Same author

Child oral health in migrant families: A cross-sectional study of caries in 1-4 year old children from migrant backgrounds residing in Melbourne, Australia.

Community dental health·2016
Same author

Hospital Drg Costing and Health Services Use of Very Pre-Term Infants From the Proprems Neuro Study Across 10 Hospitals in Australia and New Zealand.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016

Related Experiment Video

Updated: Jul 5, 2026

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Theoretical principles of in vitro selection using combinatorial nucleic acid libraries.

B Vant-Hull1, L Gold, D A Zichi

  • 1NeXstar Pharmaceuticals, Boulder, Colorado, USA.

Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
PubMed
Summary

Systematic evolution of ligands by exponential enrichment (SELEX) offers a new approach to drug discovery. This method uses nucleic acid libraries and selection rounds to identify high-affinity molecules for biological research.

More Related Videos

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Related Experiment Videos

Last Updated: Jul 5, 2026

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Discovery

Background:

  • In vitro selection techniques, such as systematic evolution of ligands by exponential enrichment (SELEX), have emerged as powerful tools.
  • These technologies integrate combinatorial chemistry with iterative selection and amplification processes.
  • SELEX enables the discovery of nucleic acid ligands with specific binding properties.

Purpose of the Study:

  • To provide an overview of SELEX technology and its applications in biological research and drug discovery.
  • To discuss the components and principles underlying the SELEX process.
  • To explore factors influencing the efficiency and success of SELEX experiments.

Main Methods:

  • Discussion of various nucleic acid libraries suitable for SELEX.
  • Analysis of affinity probability distributions to characterize ligand-target interactions.
  • Development and application of an equilibrium model for SELEX.
  • Identification of optimal experimental conditions, including reactant concentrations and signal-to-noise ratios.

Main Results:

  • The overview details the types of nucleic acid libraries that can be employed in SELEX.
  • It examines the concept of affinity probability distributions for understanding binding characteristics.
  • An equilibrium model is presented to describe the SELEX process.
  • Optimal conditions for maximizing the efficiency of SELEX are discussed.

Conclusions:

  • SELEX represents a significant advancement in identifying high-affinity ligands for diverse biological targets.
  • Understanding the theoretical underpinnings, such as affinity distributions and equilibrium models, is crucial for optimizing SELEX.
  • The systematic approach allows for the tailored selection of molecules, advancing drug discovery and biological research.