Characterization of tertiary folding of RNA by circular dichroism and urea
1University of Chicago, Chicago, Illinois, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
CD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer-controlled titrators, data can be acquired rapidly, and accurate thermodynamic properties can be obtained over a wide variety of conditions. Thus, CD spectroscopy provides a useful complement to site-resolved or chemical modification methods.
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