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Updated: Jul 5, 2026
13:41
Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
Published on: October 17, 2011
Time-resolved hydroxyl radical footprinting of RNA with X-rays
S A Woodson1, M L Deras, M Brenowitz
1Johns Hopkins University, Baltimore, Maryland, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.
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