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Updated: Jul 5, 2026

Surface Functionalization of Metal-Organic Frameworks for Improved Moisture Resistance
Published on: September 5, 2018
2'-Hydroxyl-protecting groups that are either photochemically labile or sensitive to fluoride ions
T J Miller1, M E Schwartz, G R Gough
1Purdue University, West Lafayette, Indiana, USA.
Abstract:
Protected ribonucleotide monomers are more difficult to obtain than their 2'-deoxy counterparts because of the need to protect the 2'-hydroxy function. This unit describes the stepwise preparation of suitably 2'-protected ribonucleosides using two protecting groups: 2-nitrobenzyloxymethyl (NBOM) and tert-butyldimethylsilyl (TBDMS). In addition, details are given for protecting the 5'-hydroxyl and the nucleobase, yielding nucleosides that are easily converted to phosphoramidite or H-phosphonate derivatives for automated oligoribonucleotide synthesis.
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