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Updated: Jul 5, 2026
13:19
Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Deoxyribo- and ribonucleoside H-phosphonates
1Stockholm University, Stockholm, Sweden.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
Most methods for preparing H-phosphonate monoesters suffer from variable yields and are often incompatible with common protecting groups used in oligonucleotide synthesis. This unit describes four procedures that consistently give high yields of the desired products. Taken together, they provide an arsenal of phosphonylation procedures that it compatible with most common protecting groups.
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Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
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