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Updated: Jul 5, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

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Published on: April 3, 2014

Attachment of nucleosides to solid-phase supports.

¹University of Calgary, Calgary, Alberta, Canada.

Current Protocols in Nucleic Acid Chemistry

|April 23, 2008

Summary

This study details methods for attaching nucleosides to solid supports using specific linker arms, enabling efficient synthesis of custom oligonucleotides for various applications.

Area of Science:

Oligonucleotide Synthesis
Solid-Phase Chemistry
Bioconjugation

Background:

Solid-phase synthesis is crucial for creating custom DNA and RNA sequences.
Choosing appropriate linker strategies is essential for efficient and successful oligonucleotide synthesis.
Long-chain alkylamine-coupled controlled-pore glass (LCAA-CPG) is a common solid support material.

Purpose of the Study:

To provide detailed protocols for coupling nucleosides to LCAA-CPG supports.
To compare the utility of succinic acid and hydroquinone-O,O'-diacetic acid linkers for oligonucleotide synthesis.
To offer guidelines for selecting optimal linker arms and coupling protocols for diverse applications.

Main Methods:

Step-by-step instructions for nucleoside coupling to LCAA-CPG supports.

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Detailed protocols for using succinic acid and hydroquinone-O,O'-diacetic acid linkers.

Guidelines for linker arm selection and coupling protocol optimization.

Main Results:

Established protocols for nucleoside attachment using two distinct linker arms.
Highlighted the widespread use and availability of the succinic acid linker.
Demonstrated the advantages of the hydroquinone-O,O'-diacetic acid linker for base-sensitive modifications and high throughput due to milder cleavage conditions.

Conclusions:

The described linker arms and protocols are versatile and can satisfy most synthetic oligonucleotide requirements.
The choice of linker arm impacts compatibility with sequence modifications and synthetic efficiency.
These methods facilitate the production of custom oligonucleotides for a broad range of research and diagnostic applications.