Oligoribonucleotides with 2'-O-(tert-butyldimethylsilyl) groups
1Ribozyme Pharmaceuticals, Boulder, Colorado, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
The chemical synthesis of oligoribonucleotides on solid support is routinely performed via the phosphoramidite method. However, the additional 2-OH function of the ribofuranosyl sugar requires suitable protection during oligoribonucleotide synthesis. This unit describes methods for 2-OH protection using the TBDMS group.
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