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Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides
1Colorado State University, Fort Collins, Colorado, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid-phase oligonucleotide synthesis supports. However, 3-oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.
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