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Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Synthesis and purification of oligonucleotide N3'-->P5' phosphoramidates and their phosphodiester and
1Lynx Therapeutics, Hayward, California, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
This unit describes the synthesis and purification of oligonucleotide N3'-->P5' phosphoramidates, wherein each 3'-oxygen is replaced by a 3'-amine in the 2'-deoxyribose ring. The synthesis of required monomers and application of the method to preparation of phosphodiester- and phosphorothioate-containing chimera of phosphoramidate is also reported.
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