Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

Applications Of NMR In Biology

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids and Nucleotides

Nucleic Acids and Nucleotides

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria. In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Force unfolding kinetics of RNA using optical tweezers. II. Modeling experiments.

Biophysical journal·2007

Same author

Verification of the Crooks fluctuation theorem and recovery of RNA folding free energies.

Nature·2005

Same author

The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.

Biochemical Society transactions·2004

Same author

[A 65-year-old woman with mediastinal enlargement].

Revista clinica espanola·2004

Same author

Efficacy of directly observed treatment of HIV infection: experience in AIDS welfare homes.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2004

Same author

A two-state kinetic model for the unfolding of single molecules by mechanical force.

Proceedings of the National Academy of Sciences of the United States of America·2002

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Biophysical analysis of nucleic acids.

¹University of California, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Current Protocols in Nucleic Acid Chemistry

|April 23, 2008

Summary

This summary is machine-generated.

This overview compares biophysical methods like X-ray diffraction and nuclear magnetic resonance for molecular structure analysis. It details the pros and cons of each technique, aiding method selection.

More Related Videos

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Related Experiment Videos

Last Updated: Jul 5, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Area of Science:

Biophysics
Structural Biology
Biochemistry

Background:

Understanding molecular structures is crucial in biological sciences.
Various biophysical techniques exist for structure determination.
A comparative analysis of these methods is needed.

Purpose of the Study:

To provide a comprehensive overview of key biophysical methods for structure analysis.
To compare the advantages and disadvantages of different techniques.
To aid researchers in selecting appropriate methods for their studies.

Main Methods:

X-ray diffraction
Nuclear Magnetic Resonance (NMR) spectroscopy
Optical spectroscopy
Theoretical and computational modeling
Single-molecule analysis

Main Results:

Each method offers unique insights into molecular structure.
X-ray diffraction provides high-resolution static structures.
NMR excels in studying molecular dynamics and solution structures.
Spectroscopic and computational methods offer complementary information.
Single-molecule techniques allow for the study of heterogeneity.

Conclusions:

The choice of method depends on the specific research question and sample.
Combining multiple techniques often yields a more complete structural picture.
Continued development of biophysical methods enhances structural biology research.