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Updated: Jul 5, 2026

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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
Published on: May 3, 2014
Methods to crystallize RNA
A R Ferré-D'Amaré1, J A Doudna
1Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.

