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Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

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Related Experiment Video

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Engineering terminal disulfide bonds into DNA.

¹University of Michigan, Ann Arbor, Michigan, USA.

Current Protocols in Nucleic Acid Chemistry

|April 23, 2008

Summary

This study introduces a straightforward method for creating disulfide cross-links in nucleic acids. These cross-links are valuable for investigating DNA and RNA structure, dynamics, and function.

Area of Science:

Molecular Biology
Biochemistry
Chemical Biology

Background:

Proteins are studied using cystine cross-links.
Nucleic acid structure and function require advanced probing techniques.

Purpose of the Study:

To develop a general method for modifying nucleic acids with disulfide cross-links.
To enable detailed studies of nucleic acid structure, dynamics, thermodynamics, folding, and function.

Main Methods:

A simple protocol for introducing disulfide cross-links into nucleic acids.
Mild air oxidation for quantitative cross-link formation.
Synthesis of various disulfide-cross-linked nucleic acid structures.

Main Results:

Successfully synthesized disulfide-cross-linked DNA and RNA structures, including hairpins, duplexes, triplexes, and tRNAs.

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Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

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Demonstrated that cross-links form quantitatively under mild conditions.

Showed that disulfide cross-links do not perturb nucleic acid secondary or tertiary structure.

Conclusions:

Disulfide cross-linking is a versatile and non-perturbing method for studying nucleic acids.
This technique provides a powerful new tool for nucleic acid research.
The method is applicable to a wide range of nucleic acid structures and conformations.