Synthesis of 2'-O-beta-d-Ribofuranosylnucleosides
Sergey N Mikhailov1, Ekaterina V Efimtseva, Andrei A Rodionov
1Russian Academy of Sciences, Engelhardt Institute of Molecular Biology, Moscow, Russia.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
A simple and efficient method for the preparation of 2-O-beta-D-ribofuranosylnucleosides, minor tRNA components, is described in this unit. The method consists of condensation of a small excess of 1-O-acetyl-2,3,5-tri-O-benzoyl-beta-D-ribofuranose activated with tin tetrachloride with N-protected 3,5-O-tetra-isopropyldisiloxane-1,3-diyl-ribonucleosides in 1,2-dichloroethane. Subsequent deprotection produces 2-O-beta-D-ribofuranosylnucleosides in an overall yield of 46% to 72%.
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