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Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jul 5, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Agarose gel electrophoresis.

D Voytas1

  • 1Iowa State University, Ames, Iowa, USA.

Current Protocols in Protein Science
|April 23, 2008
PubMed
Summary

This study details a method for isolating and purifying DNA fragments ranging from 0.5 to 25 kilobases (kb). The protocol utilizes midigels and minigels for efficient separation and purification of these DNA segments.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Accurate DNA fragment separation is crucial for molecular biology techniques.
  • Existing methods may have limitations in range or efficiency.
  • Standardized protocols are needed for reproducible results.

Purpose of the Study:

  • To present a reliable protocol for DNA fragment separation and purification.
  • To cover a broad size range of DNA fragments (0.5–25 kb).
  • To detail the application of midigel and minigel electrophoresis.

Main Methods:

  • Development of a protocol for DNA fragment isolation.
  • Application of gel electrophoresis using midigel and minigel formats.
  • Purification of DNA fragments post-electrophoresis.

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One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

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Agarose Gel Electrophoresis for the Separation of DNA Fragments
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One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
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One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

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Main Results:

  • Successful separation of DNA fragments within the 0.5–25 kb range.
  • Demonstration of purification efficiency for targeted DNA fragments.
  • Validation of the protocol using both midigel and minigel systems.

Conclusions:

  • The presented protocol offers an effective method for DNA fragment separation and purification.
  • The use of midigels and minigels provides flexibility for different experimental needs.
  • This protocol supports downstream molecular biology applications requiring specific DNA fragment sizes.